Control of adenosine deaminase levels in human lymphoblasts
نویسندگان
چکیده
منابع مشابه
Characteristics of an aminohydrolase distinct from adenosine deaminase in cultured human lymphoblasts.
An inherited deficiency of adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) is associated with an autosomal recessive form of severe combined immunodeficiency disease. Affected patients exhibit markedly reduced or absent adenosine deaminating activity in various tissues. In this study we have demonstrated the presence of a low level aminohydrolase activity in 11 different normal and a...
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Human erythrocyte adenosine deaminase has been purified approximately 800,000-fold to apparent homogeneity using antibody affinity chromatography. The enzyme was shown to be a single polypeptide chain with an estimated molecular weight of approximately 38,000. The three electrophoretic forms of erythrocyte adenosine deaminase purified simultaneoudy by this technique were indistinguishable by so...
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The diagnosis of organ disease is aided by measurement of number of nonfunctional plasma enzymes characteristic of that organ/tissue. The amount of enzymes released depends on the degree of cellular damage, the intra-cellular concentration of enzymes, and the mass of the effected tissue. The concentration of enzyme released reflects the severity of damage. Once the presence of hepatic dysfuncti...
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In many human tissues adenosine deaminase exists as a complex composed of two proteins; one protein has adenosine deaminase activity while the other represents a binding protein with no other known binding activity. A rapid, quantitative assay for human adenosine deaminase binding protein has been developed utilizing ‘251-labeled calf adenosine deaminase. In addition this binding protein has be...
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We have treated a patient with refractory T-cell acute lymphoblastic leukemia with 2'-deoxycoformycin, a potent inhibitor of the enzyme adenosine deaminase. Inhibition of adenosine deaminase activity resulted in (1) an abrupt rise in plasma deoxyadenosine, but not adenosine, concentrations; (2) accumulation of deoxyadenosine triphosphate by lymphoblasts; (3) inhibition of the enzyme S-adenoylho...
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ژورنال
عنوان ژورنال: Advances in Enzyme Regulation
سال: 1982
ISSN: 0065-2571
DOI: 10.1016/0065-2571(82)90014-0